Load mouse ESCs raw count matrix | Load in vivo mouse datasets | Compute markers for selected in vivo stages | Select only black/white markers for in vivo stages | Run SCOPRO | Visualization of conserved/ not conserved genes between late 2 cells stage and in vitro clusters

Load human gastrula data and create norm counts and knn matrices using Seurat | Run CIARA | Identifying highly localized genes | Cluster analysis using CIARA highly localized genes | Sub-cluster analysis using CIARA | CIARA identifies three rare population of cells with highly distinctive transcriptional profile